ARG54882

anti-RUNX2 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μl

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes RUNX2
反应物种	Hu
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	RUNX2
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 445-474 surrounding S533 of Human RUNX2.
偶联标记	Un-conjugated
別名	OSF2; Core-binding factor subunit alpha-1; CLCD; AML3; Acute myeloid leukemia 3 protein; Polyomavirus enhancer-binding protein 2 alpha A subunit; CBFA1; SL3/AKV core-binding factor alpha A subunit; PEA2aA; Osteoblast-specific transcription factor 2; SL3-3 enhancer factor 1 alpha A subunit; PEBP2-alpha A; Oncogene AML-3; CBF-alpha-1; CCD; PEBP2aA; CCD1; OSF-2; PEA2-alpha A; Runt-related transcription factor 2

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
IHC-P	Assay-dependent
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

CEM

属性

形式	Liquid
纯化	Purification with Protein A and immunogen peptide.
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 860 Human RUNX2 Swiss-port # Q13950 Human Runt-related transcription factor 2
基因名称	RUNX2
全名	runt-related transcription factor 2
背景介绍	This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]
生物功能	Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation. [UniProt]
细胞定位	Nucleus.
研究领域	Developmental Biology antibody; Gene Regulation antibody
预测分子量	57 kDa
翻译后修饰	Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation (By similarity). Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.

检测图片 (3) Click the Picture to Zoom In

ARG54882 anti-RUNX2 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG54882 anti-RUNX2 antibody.
ARG54882 anti-RUNX2 antibody WB image

Western blot: 35 µg of CEM cell lysate stained with ARG54882 anti-RUNX2 antibody.
ARG54882 anti-RUNX2 antibody FACS image

Flow Cytometry: NCI-H460 cells stained with ARG54882 anti-RUNX2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.