ARG41230
anti-RUFY1 antibody
anti-RUFY1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes RUFY1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RUFY1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human RUFY1. (C-QKNEAITSFEGKT) (NP_079434.3; NP_001035541.1) |
| 偶联标记 | Un-conjugated |
| 別名 | FYVE-finger protein EIP1; RUN and FYVE domain-containing protein 1; ZFYVE12; Zinc finger FYVE domain-containing protein 12; Rab4-interacting protein; La-binding protein 1; RABIP4 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 80 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96T51 Human RUN and FYVE domain-containing protein 1 |
|---|---|
| 基因名称 | RUFY1 |
| 全名 | RUN and FYVE domain containing 1 |
| 背景介绍 | This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| 生物功能 | Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. [UniProt] |
| 细胞定位 | Cytoplasm. Early endosome membrane; Peripheral membrane protein. [UniProt] |
| 预测分子量 | 80 kDa |
| 翻译后修饰 | Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41230 anti-RUFY1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue stained with ARG41230 anti-RUFY1 antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
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ARG41230 anti-RUFY1 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG41230 anti-RUFY1 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
