ARG42178
anti-RTCB / HSPC117 antibody
anti-RTCB / HSPC117 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes RTCB / HSPC117 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RTCB / HSPC117 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human RTCB / HSPC117. (C-QADPNKVSARAKKR) (NP_055121.1) |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.5.1.3; HSPC117; DJ149A16.6; C22orf28; tRNA-splicing ligase RtcB homolog; FAAP |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 55 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RTCB |
| 全名 | RNA 2',3'-cyclic phosphate and 5'-OH ligase |
| 生物功能 | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low. [UniProt] |
| 预测分子量 | 55 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42178 anti-RTCB / HSPC117 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42178 anti-RTCB / HSPC117 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42178 anti-RTCB / HSPC117 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG42178 anti-RTCB / HSPC117 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
