ARG59083
anti-RRM2 antibody
anti-RRM2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RRM2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | This antibody might cross-react to RRM2B protein based on sequence analysis. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RRM2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-389 of Human RRM2 (NP_001025.1). |
| 偶联标记 | Un-conjugated |
| 別名 | R2; EC 1.17.4.1; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RR2M; RR2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Mouse thymus | ||||||||||
| 实际分子量 | 45kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RRM2 |
| 全名 | ribonucleotide reductase M2 |
| 背景介绍 | This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009] |
| 生物功能 | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59083 anti-RRM2 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG59083 anti-RRM2 antibody.
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ARG59083 anti-RRM2 antibody WB image
Western blot: 25 µg of Mouse thymus lysate stained with ARG59083 anti-RRM2 antibody at 1:1000 dilution.
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ARG59083 anti-RRM2 antibody IP image
Immunoprecipitation: 200 µg extracts of HeLa cells were immunoprecipitated and stained with ARG59083 anti-RRM2 antibody at 1:1000 dilution.
