anti-RRM1 antibody [SQab20199]

anti-RRM1 antibody [SQab20199] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat



产品描述 Recombinant Rabbit Monoclonal antibody [SQab20199] recognizes RRM1
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab20199
同位型 IgG
靶点名称 RRM1
抗原物种 Human
抗原 Synthetic peptide within aa. 700-800 of Human RRM1.
偶联标记 Un-conjugated
別名 Ribonucleoside-diphosphate reductase large subunit; R1; EC; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; RIR1; RR1


应用 推荐稀释比
IHC-P1:100 - 1:200
WB1:500 - 1:1000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
WB: for mouse and rat samples, maybe more antibody is needed, and mouse and rat samples were tested only by WB.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Breast carcinoma tissue.
实际分子量 90 kDa


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 20133 Mouse RRM1

GeneID: 6240 Human RRM1

Swiss-port # P07742 Mouse Ribonucleoside-diphosphate reductase large subunit

Swiss-port # P23921 Human Ribonucleoside-diphosphate reductase large subunit

基因名称 RRM1
全名 ribonucleotide reductase M1
背景介绍 This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
生物功能 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. [UniProt]
细胞定位 Cytoplasm. [UniProt]
预测分子量 90 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG66766 anti-RRM1 antibody [SQab20199] WB image (Customer's Feedback)

    Western blot: MCF7 cell lysate, HeLa cell lysate, Mouse heart lysate and Rat heart lysate stained with ARG66766 anti-RRM1 antibody [SQab20199] at 1:1000 dilution.

  • ARG66766 anti-RRM1 antibody [SQab20199] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human breast carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66766 anti-RRM1 antibody [SQab20199] at 18°C - 25°C for 30 minutes.


nuts_pic      Excellent

anti-RRM1 antibody [SQab20199]


Sample:MCF7, HeLa, Mouse heart, Rat heart

Sample Loading Amount:20 μg

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP