ARG55465
anti-RRM1 antibody
anti-RRM1 antibody for ICC/IF,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RRM1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RRM1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human RRM1 (NP_001024.1) |
| 偶联标记 | Un-conjugated |
| 別名 | Ribonucleoside-diphosphate reductase large subunit; R1; EC 1.17.4.1; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; RIR1; RR1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P07742 Mouse Ribonucleoside-diphosphate reductase large subunit Swiss-port # P23921 Human Ribonucleoside-diphosphate reductase large subunit |
|---|---|
| 基因名称 | RRM1 |
| 全名 | ribonucleotide reductase M1 |
| 背景介绍 | This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq, Jul 2008] |
| 生物功能 | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 90 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55465 anti-RRM1 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55465 anti-RRM1 antibody at 1:50 dilution. Left: primary antibody (red). Right: DAPI (blue).
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
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ARG55465 anti-RRM1 antibody WB image
Western blot: 30 µg of HeLa and SW480 cell lysates stained with ARG55465 anti-RRM1 antibody at 1:500 dilution.
客户反馈
Excellent
anti-RRM1 antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-RRM1 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
anti-RRM1 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
