ARG40974
anti-RPS4X / SCAR antibody
anti-RPS4X / SCAR antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RPS4X / SCAR |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RPS4X / SCAR |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 209-243 of Human RPS4X. |
| 偶联标记 | Un-conjugated |
| 別名 | 40S ribosomal protein S4, X isoform; DXS306; S4; Single copy abundant mRNA protein; RPS4; SCR10; SCAR; CCG2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HL-60 | ||||||
| 实际分子量 | 30 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P62701 Human 40S ribosomal protein S4, X isoform Swiss-port # P62702 Mouse 40S ribosomal protein S4, X isoform |
|---|---|
| 基因名称 | RPS4X |
| 全名 | ribosomal protein S4, X-linked |
| 背景介绍 | Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| 细胞定位 | Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. [UniProt] |
| 预测分子量 | 30 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG40974 anti-RPS4X / SCAR antibody WB image
Western blot: 20 µg of HL-60 cell lysate stained with ARG40974 anti-RPS4X / SCAR antibody at 1:2000 dilution.
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ARG40974 anti-RPS4X / SCAR antibody FACS image
Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by ARG40974 anti-RPS4X / SCAR antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
