ARG45802

anti-RPN2 antibody

anti-RPN2 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes RPN2
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RPN2
抗原物种 Human
抗原 Recombinant protein containing to human RPN2.
偶联标记 Un-conjugated
別名 RPN2; RPNII; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; RPN-II; RIBIIR; EC 2.4.99.18; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; Ribophorin-2; SWP1; Ribophorin II

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 69 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6185 Human RPN2

Swiss-port # P04844 Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

基因名称 RPN2
全名 ribophorin II
背景介绍 This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
生物功能 Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. [UniProt]
细胞定位 Endoplasmic reticulum. Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
预测分子量 69 kDa
翻译后修饰 Glycoprotein; Isopeptide bond; Ubl conjugation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45802 anti-RPN2 antibody ICC/IF image

    Immunofluorescence: U87 stained with ARG45802 anti-RPN2 antibody at 5 μg/ml dilution.

  • ARG45802 anti-RPN2 antibody WB image

    Western blot: THP-1 stained with ARG45802 anti-RPN2 antibody at 0.5 μg/ml dilution.

  • ARG45802 anti-RPN2 antibody FACS image

    Flow Cytometry: HL-60 stained with ARG45802 anti-RPN2 antibody at 1 µg/10^6 cells dilution.