ARG40042

anti-RPLP0 antibody

anti-RPLP0 antibody for ICC/IF,Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes RPLP0
反应物种 Hu, Ms
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RPLP0
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-317 of Human RPLP0 (NP_000993.1).
偶联标记 Un-conjugated
別名 P0; L10E; 60S acidic ribosomal protein P0; LP0; RPP0; 60S ribosomal protein L10E; PRLP0

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse thymus and OVCAR3
实际分子量 37 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11837 Mouse RPLP0

GeneID: 6175 Human RPLP0

Swiss-port # P05388 Human 60S acidic ribosomal protein P0

Swiss-port # P14869 Mouse 60S acidic ribosomal protein P0

基因名称 RPLP0
全名 ribosomal protein, large, P0
背景介绍 Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
生物功能 Ribosomal protein P0 is the functional equivalent of E.coli protein L10. [UniProt]
细胞定位 Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:19188445, PubMed:17289661). [UniProt]
预测分子量 34 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG40042 anti-RPLP0 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG40042 anti-RPLP0 antibody.

  • ARG40042 anti-RPLP0 antibody WB image

    Western blot: 25 µg of Mouse thymus and OVCAR3 cell lysates stained with ARG40042 anti-RPLP0 antibody at 1:1000 dilution.