ARG40701

anti-RPL19 antibody

anti-RPL19 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes RPL19
反应物种 Hu, Ms, Rat
预测物种 Bov, Chk, Dog, Mk, Rb
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RPL19
抗原物种 Human
抗原 Synthetic peptide corresponding to aa. 132-170 of Human RPL19. (FKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEAR)
偶联标记 Un-conjugated
別名 60S ribosomal protein L19; L19

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF1:200 - 1:1000
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 19921 Mouse RPL19

GeneID: 6143 Human RPL19

GeneID: 81767 Rat RPL19

基因名称 RPL19
全名 ribosomal protein L19
背景介绍 Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L19E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
预测分子量 23 kDa
翻译后修饰 Citrullinated by PADI4. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG40701 anti-RPL19 antibody ICC/IF image

    Immunofluorescence: U20S cells stained with ARG40701 anti-RPL19 antibody at 2 µg/ml, overnight at 4°C. Cells was counterstained with DAPI (blue).

  • ARG40701 anti-RPL19 antibody FACS image

    Flow Cytometry: U20S cells were blocked with 10% normal goat serum and stained with ARG40701 anti-RPL19 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG40701 anti-RPL19 antibody FACS image

    Flow Cytometry: A431 cells were blocked with 10% normal goat serum and stained with ARG40701 anti-RPL19 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG40701 anti-RPL19 antibody WB image

    Western blot: Rat liver, HEPA and HepG2 whole cell lysates stained with ARG40701 anti-RPL19 antibody at 0.5 µg/ml.