概述

产品描述	Rabbit Polyclonal antibody recognizes RPE65
反应物种	Hu, Ms, Rat
应用	IP, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	RPE65
抗原物种	Human
抗原	Synthetic peptide of Human RPE65.
偶联标记	Un-conjugated
別名	Retinal pigment epithelium-specific 65 kDa protein; rd12; EC 3.1.1.64; LCA2; Retinol isomerase; sRPE65; RP20; BCO3; All-trans-retinyl-palmitate hydrolase; mRPE65; Retinoid isomerohydrolase

应用说明

应用建议	应用 推荐稀释比 IP 1:50 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	Mouse eyeball
实际分子量	~ 61 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 19892 Mouse RPE65 GeneID: 6121 Human RPE65 GeneID: 89826 Rat RPE65 Swiss-port # O70276 Rat Retinoid isomerohydrolase Swiss-port # Q16518 Human Retinoid isomerohydrolase Swiss-port # Q91ZQ5 Mouse Retinoid isomerohydrolase More
基因名称	RPE65
全名	retinal pigment epithelium-specific protein 65kDa
背景介绍	This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq, Jul 2008]
生物功能	Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. [UniProt]
细胞定位	Cytoplasm. Cell membrane; Lipid-anchor. Microsome membrane. Note=Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells. [UniProt]
预测分子量	61 kDa
翻译后修饰	Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). [UniProt]

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