ARG41610

anti-RPA2 / RPA32 antibody [RPPA-1]

anti-RPA2 / RPA32 antibody [RPPA-1] for Western blot and Human

概述

产品描述 Mouse Monoclonal antibody [RPPA-1] recognizes RPA2 / RPA32
反应物种 Hu
应用 WB
宿主 Mouse
克隆 Monoclonal
克隆号 RPPA-1
同位型 IgG2b, kappa
靶点名称 RPA2 / RPA32
抗原物种 Human
抗原 Full length recombinant Human RPA2 / RPA32 protein.
偶联标记 Un-conjugated
別名 RF-A protein 2; RPA32; RP-A p34; Replication protein A 34 kDa subunit; Replication factor A protein 2; Replication protein A 32 kDa subunit; REPA2; RP-A p32; RFA2

应用说明

应用建议
应用 推荐稀释比
WB1 - 2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 30 kDa

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6118 Human RPA2

Swiss-port # P15927 Human Replication protein A 32 kDa subunit

基因名称 RPA2
全名 replication protein A2, 32kDa
生物功能 As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. [UniProt]
细胞定位 Nucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. [UniProt]
预测分子量 29 kDa
翻译后修饰 Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.

DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG41610 anti-RPA2 / RPA32 antibody [RPPA-1] WB image

    Western blot: A375 cell lysate stained with ARG41610 anti-RPA2 / RPA32 antibody [RPPA-1].