ARG66943
anti-ROS1 antibody [SQab22273]
anti-ROS1 antibody [SQab22273] for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab22273] recognizes ROS1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab22273 |
| 同位型 | IgG |
| 靶点名称 | ROS1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein fragment of Human ROS1. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Proto-oncogene tyrosine-protein kinase ROS |
| 別名 | Proto-oncogene c-Ros-1; MCF3; c-ros-1; Receptor tyrosine kinase c-ros oncogene 1; Proto-oncogene tyrosine-protein kinase ROS; c-Ros receptor tyrosine kinase; ROS; EC 2.7.10.1; Proto-oncogene c-Ros |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human ROS1+ lung carcinoma tissue |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05%BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08922 Human Proto-oncogene tyrosine-protein kinase ROS |
|---|---|
| 基因名称 | ROS1 |
| 全名 | ROS proto-oncogene 1 , receptor tyrosine kinase |
| 背景介绍 | This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008] |
| 生物功能 | Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 264 kDa |
| 翻译后修饰 | Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11. |
检测图片 (1) Click the Picture to Zoom In
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ARG66943 anti-ROS1 antibody [SQab22273] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human ROS1+ lung carcinoma tissue stained with ARG66943 anti-ROS1 antibody [SQab22273]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).
