ARG56310
anti-ROCK2 antibody
anti-ROCK2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
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概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ROCK2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ROCK2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human ROCK2 |
| 偶联标记 | Un-conjugated |
| 別名 | Rho-associated, coiled-coil-containing protein kinase II; p164 ROCK-2; ROCK-II; EC 2.7.11.1; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated protein kinase 2; Rho kinase 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | U87 | ||||||||
| 实际分子量 | 180 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ROCK2 |
| 全名 | Rho-associated, coiled-coil containing protein kinase 2 |
| 背景介绍 | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008] |
| 生物功能 | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. [UniProt] |
| 预测分子量 | 161 kDa |
| 翻译后修饰 | Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity. Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
检测图片 (3) Click the Picture to Zoom In
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ARG56310 anti-ROCK2 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG56310 anti-ROCK2 antibody.
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ARG56310 anti-ROCK2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG56310 anti-ROCK2 antibody at 1:100 dilution.
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ARG56310 anti-ROCK2 antibody WB image
Western blot: U87 cell lysate stained with ARG56310 anti-ROCK2 antibody.
客户反馈
Excellent
anti-ROCK2 antibody
Application：WB
Sample：HeLa and U2OS
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
