ARG45306

anti-RNase H1 / RNH1 antibody

anti-RNase H1 / RNH1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse

概述

产品描述 Polyclonal antibody recognizes RNase H1 / RNH1
反应物种 Hu, Ms
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RNase H1 / RNH1
抗原物种 Human
抗原 Recombinant protein containing to human RNase H1 / RNH1.
偶联标记 Un-conjugated
別名 RNASEH1; Ribonuclease H1; Ribonuclease H Type II; RNase H1; RNH1; EC 3.1.26.4; H1RNA; PEOB2

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 38 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 246243 Human RNASEH1

Swiss-port # O60930 Human Ribonuclease H1

基因名称 RNASEH1
全名 Ribonuclease H1
背景介绍 This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017]
生物功能 Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. [UniProt]
细胞定位 Cytoplasm. [UniProt]
预测分子量 32 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG45306 anti-RNase H1 / RNH1 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45306 anti-RNase H1 / RNH1 antibody at 5 ug/ml dilution.

  • ARG45306 anti-RNase H1 / RNH1 antibody WB image

    Western blot: Hela and SH-SY5Y stained with ARG45306 anti-RNase H1 / RNH1 antibody at 0.5 μg/ml dilution.

  • ARG45306 anti-RNase H1 / RNH1 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45306 anti-RNase H1 / RNH1 antibody at 1 µg/10^6 cells dilution.