ARG41178
anti-RNF40 antibody
anti-RNF40 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RNF40 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RNF40 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-300 of Human RNF40 (NP_055586.1). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; RBP95; BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein; RING finger protein 40; E3 ubiquitin-protein ligase BRE1B; STARING |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HepG2 | ||||
| 实际分子量 | 140 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RNF40 |
| 全名 | ring finger protein 40, E3 ubiquitin protein ligase |
| 背景介绍 | The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011] |
| 生物功能 | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 114 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG41178 anti-RNF40 antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG41178 anti-RNF40 antibody at 1:1000 dilution.
