ARG43158
anti-RNF125 antibody
anti-RNF125 antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RNF125 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RNF125 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-105 of Human RNF125 (NP_060301.2). |
| 偶联标记 | Un-conjugated |
| 別名 | RING finger protein 125; EC 6.3.2.-; T-cell RING activation protein 1; E3 ubiquitin-protein ligase RNF125; TRAC1; TRAC-1; TNORS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | K562 | ||||
| 实际分子量 | ~ 28 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96EQ8 Human E3 ubiquitin-protein ligase RNF125 Swiss-port # Q9D9R0 Mouse E3 ubiquitin-protein ligase RNF125 |
|---|---|
| 基因名称 | RNF125 |
| 全名 | ring finger protein 125, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012] |
| 生物功能 | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed:15843525, PubMed:17460044, PubMed:17643463, PubMed:26027934, PubMed:26471729, PubMed:25591766, PubMed:27411375). Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation (PubMed:17460044, PubMed:26471729). Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed:25591766). Mediates ubiquitination and subsequent degradation of JAK1 (PubMed:26027934). Acts as a positive regulator of T-cell activation (PubMed:15843525). [UniProt] |
| 细胞定位 | Golgi apparatus membrane; Lipid-anchor. Note=Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Autoubiquitinated, leading to its subsequent proteasomal degradation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG43158 anti-RNF125 antibody WB image
Western blot: 25 µg of K562 cell lysate stained with ARG43158 anti-RNF125 antibody at 1:1000 dilution.
