ARG43096
anti-RNF123 antibody
anti-RNF123 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RNF123 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RNF123 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to R12-Y188 of Human RNF123. |
| 偶联标记 | Un-conjugated |
| 別名 | KPC1; EC 6.3.2.-; RING finger protein 123; E3 ubiquitin-protein ligase RNF123; FP1477; Kip1 ubiquitination-promoting complex protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 160 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q5XPI3 Mouse E3 ubiquitin-protein ligase RNF123 Swiss-port # Q5XPI4 Human E3 ubiquitin-protein ligase RNF123 |
|---|---|
| 基因名称 | RNF123 |
| 全名 | ring finger protein 123 |
| 背景介绍 | The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| 生物功能 | Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Promotes the ubiquitination and proteasome-mediated degradation of CDKN1B which is the cyclin-dependent kinase inhibitor at the G0-G1 transition of the cell cycle (PubMed:15531880, PubMed:16227581). Functions also as an inhibitor of innate antiviral signaling mediated by DDX58 and IFIH1 independently of its E3 ligase activity (PubMed:27312109). Interacts with the N-terminal CARD domains of DDX58 and IFIH1 and competes with the downstream adapter MAVS (PubMed:27312109). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 149 kDa |
| 翻译后修饰 | Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
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ARG43096 anti-RNF123 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43096 anti-RNF123 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43096 anti-RNF123 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta, THP-1, HepG2, PC-3, U-87MG, K562 and HeLa whole cell lysates stained with ARG43096 anti-RNF123 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43096 anti-RNF123 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43096 anti-RNF123 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43096 anti-RNF123 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43096 anti-RNF123 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43096 anti-RNF123 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43096 anti-RNF123 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43096 anti-RNF123 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43096 anti-RNF123 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43096 anti-RNF123 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat testis, Mouse testis and HEPA1-6 whole cell lysates stained with ARG43096 anti-RNF123 antibody at 0.5 µg/ml dilution, overnight at 4°C.
