ARG58229
anti-RHOH antibody
anti-RHOH antibody for ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RHOH |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RHOH |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-191 of Human RHOH (NP_004301.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Translocation three four protein; GTP-binding protein TTF; Rho-related GTP-binding protein RhoH; TTF; ARHH |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HT-29 | ||||||
| 实际分子量 | 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q15669 Human Rho-related GTP-binding protein RhoH Swiss-port # Q9D3G9 Mouse Rho-related GTP-binding protein RhoH |
|---|---|
| 基因名称 | RHOH |
| 全名 | ras homolog family member H |
| 背景介绍 | The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013] |
| 生物功能 | Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. [UniProt] |
| 细胞定位 | Cytoplasm, Cell membrane, Lipid-anchor, Cytoplasmic side. [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development (By similarity). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58229 anti-RHOH antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG58229 anti-RHOH antibody.
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ARG58229 anti-RHOH antibody WB image
Western blot: 25 µg of HT-29 cell lysate stained with ARG58229 anti-RHOH antibody at 1:1000 dilution.
