ARG58777
anti-RFX5 antibody
anti-RFX5 antibody for Western blot and Human,Mouse
概述
| 产品描述 | Goat Polyclonal antibody recognizes RFX5 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RFX5 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the internal region of Human RFX5 (NP_000440.1). (C-TGDKSSEPSTLSNE) |
| 偶联标记 | Un-conjugated |
| 別名 | DNA-binding protein RFX5; Regulatory factor X 5 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 73 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RFX5 |
| 全名 | regulatory factor X, 5 (influences HLA class II expression) |
| 背景介绍 | A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. [UniProt] |
| 预测分子量 | 65 kDa |
| 翻译后修饰 | Phosphorylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58777 anti-RFX5 antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG58777 anti-RFX5 antibody at 2 µg/ml and incubated for 1 hour at RT.
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ARG58777 anti-RFX5 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG58777 anti-RFX5 antibody at 2 µg/ml and incubated for 1 hour at RT.
