ARG41294
anti-RFC2 antibody
anti-RFC2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RFC2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Bov |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RFC2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 17-46 of Human RFC2. |
| 偶联标记 | Un-conjugated |
| 別名 | Activator 1 subunit 2; Activator 1 40 kDa subunit; A1 40 kDa subunit; Replication factor C subunit 2; RFC40; Replication factor C 40 kDa subunit; RF-C 40 kDa subunit |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | K562 | ||||||||
| 实际分子量 | 39 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RFC2 |
| 全名 | replication factor C (activator 1) 2, 40kDa |
| 背景介绍 | This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013] |
| 生物功能 | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 39 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG41294 anti-RFC2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue stained with ARG41294 anti-RFC2 antibody at 1:200 dilution and counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery.
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ARG41294 anti-RFC2 antibody WB image
Western blot: 20 ug of K562 whole cell lysate stained with ARG41294 anti-RFC2 antibody at 1:1000 dilution.
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ARG41294 anti-RFC2 antibody FACS image
Flow Cytometry: K562 cells stained with ARG41294 anti-RFC2 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
