ARG59903
anti-RFC1 antibody
anti-RFC1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RFC1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RFC1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 400-700 of Human RFC1 (NP_001191676.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Activator 1 subunit 1; RFC; Replication factor C large subunit; PO-GA; DNA-binding protein PO-GA; RF-C 140 kDa subunit; Replication factor C subunit 1; A1; RFC140; RECC1; Activator 1 large subunit; Replication factor C 140 kDa subunit; A1 140 kDa subunit; Activator 1 140 kDa subunit; MHCBFB |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | MCF7 | ||||||
| 实际分子量 | 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RFC1 |
| 全名 | replication factor C (activator 1) 1, 145kDa |
| 背景介绍 | This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011] |
| 生物功能 | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 128 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG59903 anti-RFC1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG59903 anti-RFC1 antibody.
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ARG59903 anti-RFC1 antibody WB image
Western blot: 25 µg of MCF7 cell lysate stained with ARG59903 anti-RFC1 antibody at 1:500 dilution.
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ARG59903 anti-RFC1 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG59903 anti-RFC1 antibody.
