ARG45300
anti-RBM25 antibody
anti-RBM25 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Polyclonal antibody recognizes RBM25 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RBM25 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human RBM25. |
| 偶联标记 | Un-conjugated |
| 別名 | RBM25; RNA Binding Motif Protein 25; FSAP94; NET52; Snu71; RNPC7; S164; Functional Spliceosome-Associated Protein 94; RNA-Binding Region (RNP1, RRM) Containing 7; RNA-Binding Region-Containing Protein 7; Arg/Glu/Asp-Rich Protein Of 120 KDa; RNA-Binding Protein 25; RED120; U1 Small Nuclear Ribonucleoprotein 1SNRP Homolog; Arg/Glu/Asp-Rich Protein, 120 KDa; RNA-Binding Motif Protein 25; Protein S164 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RBM25 |
| 全名 | RNA Binding Motif Protein 25 |
| 背景介绍 | Enables mRNA binding activity. Involved in regulation of alternative mRNA splicing, via spliceosome and regulation of apoptotic process. Located in nuclear speck. [provided by Alliance of Genome Resources, Nov 2024] |
| 生物功能 | RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.. [UniProt] |
| 细胞定位 | Cytoplasm; Nucleus. [UniProt] |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG45300 anti-RBM25 antibody WB image
Western blot: PC-2 and SiHa stained with ARG45300 anti-RBM25 antibody at 0.5 μg/ml dilution.
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ARG45300 anti-RBM25 antibody FACS image
Flow Cytometry: SiHa stained with ARG45300 anti-RBM25 antibody at 1 µg/10^6 cells dilution.
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ARG45300 anti-RBM25 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45300 anti-RBM25 antibody at 2 μg/ml dilution.
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ARG45300 anti-RBM25 antibody WB image
Western blot: RH35 and PC-12 stained with ARG45300 anti-RBM25 antibody at 0.5 μg/ml dilution.
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ARG45300 anti-RBM25 antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45300 anti-RBM25 antibody at 2 μg/ml dilution.
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ARG45300 anti-RBM25 antibody WB image
Western blot: NIH/3T3 and RAW264.7 stained with ARG45300 anti-RBM25 antibody at 0.5 μg/ml dilution.
