ARG42822
anti-RBL2 / p130 phospho (Thr986) antibody
anti-RBL2 / p130 phospho (Thr986) antibody for Flow cytometry,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RBL2 / p130 phospho (Thr986) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RBL2 / p130 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Thr986 of Human RBL2 / p130. |
| 偶联标记 | Un-conjugated |
| 別名 | RBR-2; p130; Retinoblastoma-related protein 2; Rb2; P130; Retinoblastoma-like protein 2; 130 kDa retinoblastoma-associated protein; pRb2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | K562 | ||||||||
| 实际分子量 | ~ 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RBL2 |
| 全名 | retinoblastoma-like 2 |
| 生物功能 | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 128 kDa |
| 翻译后修饰 | During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG42822 anti-RBL2 / p130 phospho (Thr986) antibody WB image
Western blot: K562 cell lysate stained with ARG42822 anti-RBL2 / p130 phospho (Thr986) antibody at 1:1000 dilution.
