ARG56914
anti-RASSF1A antibody [3F3]
anti-RASSF1A antibody [3F3] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [3F3] recognizes RASSF1A |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3F3 |
| 同位型 | IgG1, kappa |
| 靶点名称 | RASSF1A |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-340 of Human RASSF1A. |
| 偶联标记 | Un-conjugated |
| 別名 | NORE2A; RASSF1A; REH3P21; Ras association domain-containing protein 1; 123F2; RDA32 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NS23 Human Ras association domain-containing protein 1 |
|---|---|
| 基因名称 | RASSF1 |
| 全名 | Ras association (RalGDS/AF-6) domain family member 1 |
| 背景介绍 | This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011] |
| 生物功能 | Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. [UniProt] |
| 预测分子量 | 39 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG56914 anti-RASSF1A antibody [3F3] ICC/IF image
Immunoflorescense: A431 cells were stained with ARG56914 anti-RASSF1A antibody [3F3].
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ARG56914 anti-RASSF1A antibody [3F3] WB image
Western blot: 40 µg of HeLa cell lysate stained with ARG56914 anti-RASSF1A antibody [3F3] at 1:500.
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ARG56914 anti-RASSF1A antibody [3F3] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56914 anti-RASSF1A antibody [3F3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56914 anti-RASSF1A antibody [3F3] WB image
Western blot: 5 µg of 1) 293T cell lysate, 2) RASSF1A Transfected 293T cell lysate stained with ARG56914 anti-RASSF1A antibody [3F3] at 1:500.
