ARG63424
anti-RANGAP1 antibody
anti-RANGAP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes RANGAP1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 特异性 | Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RANGAP1 |
| 抗原物种 | Human |
| 抗原 | ASEDIAKLAETLAK-C |
| 偶联标记 | Un-conjugated |
| 別名 | RANGAP; Fug1; RanGAP1; Ran GTPase-activating protein 1; SD |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 64 kDa |
| 翻译后修饰 | Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. |
检测图片 (2) Click the Picture to Zoom In
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ARG63424 anti-RANGAP1 antibody WB image
Western Blot: 3T3 lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63424 anti-RANGAP1 antibody at 0.2 µg/ml dilution.
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ARG63424 anti-RANGAP1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63424 anti-RANGAP1 antibody at 2.5 µg/ml dilution followed by AP-staining.
