ARG63568
anti-RANBP1 antibody
anti-RANBP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes RANBP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RANBP1 |
| 抗原物种 | Human |
| 抗原 | C-LSVKEETKEDAEEKQ |
| 偶联标记 | Un-conjugated |
| 別名 | Ran-specific GTPase-activating protein; HTF9A; Ran-binding protein 1; RanBP1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P43487 Human Ran-specific GTPase-activating protein |
|---|---|
| 背景介绍 | Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 23 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG63568 anti-RANBP1 antibody WB image
Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG63568 anti-RANBP1 antibody at 0.05 µg/ml dilution.
-
ARG63568 anti-RANBP1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney stained with ARG63568 anti-RANBP1 antibody (3.8µg/ml). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
