ARG45520

anti-RALB antibody

anti-RALB antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes RALB
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RALB
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminal region of human RALB.
偶联标记 Un-conjugated
別名 RALB; v-ral simian leukemia viral oncogene homolog B; Ras-related protein Ral-B

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.1-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 23 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 116546 Rat RALB

GeneID: 5899 Human RALB

GeneID: 64143 Mouse RALB

基因名称 RALB
全名 v-ral simian leukemia viral oncogene homolog B
背景介绍 This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. [provided by RefSeq, Jul 2008]
生物功能 Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis. [UniProt]
细胞定位 Cell membrane. [UniProt]
预测分子量 23 kDa
翻译后修饰 Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
The farnesylated form confers resistance to the proapoptotic and anti-anchorage-dependent growth effects of geranylgeranyltransferase I inhibitors, including GGTI-2417.. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45520 anti-RALB antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45520 anti-RALB antibody at 5 μg/ml dilution.

  • ARG45520 anti-RALB antibody WB image

    Western blot: Hela stained with ARG45520 anti-RALB antibody at 0.5 μg/ml dilution.

  • ARG45520 anti-RALB antibody FACS image

    Flow Cytometry: Jurkat stained with ARG45520 anti-RALB antibody at 1 µg/10^6 cells dilution.

  • ARG45520 anti-RALB antibody WB image

    Western blot: Rat Brain stained with ARG45520 anti-RALB antibody at 0.5 μg/ml dilution.

  • ARG45520 anti-RALB antibody WB image

    Western blot: Mouse Spleen stained with ARG45520 anti-RALB antibody at 0.5 μg/ml dilution.