ARG63188
anti-RACGAP1 / MgcRacGAP antibody
anti-RACGAP1 / MgcRacGAP antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes RACGAP1 / MgcRacGAP |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Dog |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 特异性 | Reported variants represent identical protein: NP_037409.2, NP_001119576.1 and NP_001119575.1. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RACGAP1 / MgcRacGAP |
| 抗原物种 | Human |
| 抗原 | C-GRQGNFFASPMLK |
| 偶联标记 | Un-conjugated |
| 別名 | CYK4; Rac GTPase-activating protein 1; MgcRacGAP; Protein CYK4 homolog; Male germ cell RacGap; ID-GAP; HsCYK-4 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 71 kDa |
| 翻译后修饰 | Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation. |
检测图片 (6) Click the Picture to Zoom In
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ARG63188 anti-RACGAP1 / MgcRacGAP antibody WB image
Western Blot: K562 cell lysate (35 µg protein in RIPA buffer). stained with ARG63188 anti-RACGAP1 / MgcRacGAP antibody at 1 µg/ml dilution.
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ARG63188 anti-RACGAP1 / MgcRacGAP antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton. Cells were stained with ARG63188 anti-RACGAP1 / MgcRacGAP antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63188 anti-RACGAP1 / MgcRacGAP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63188 anti-RACGAP1 / MgcRacGAP antibody at 4 µg/ml dilution followed by HRP-staining.
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ARG63188 anti-RACGAP1 / MgcRacGAP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63188 anti-RACGAP1 / MgcRacGAP antibody at 1 µg/ml dilution followed by HRP-staining.
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ARG63188 anti-RACGAP1 / MgcRacGAP antibody WB image
Western blot: 35 µg of A431 nucleus (A), Jurkat (B), Jurkat nucleus (C) and Human pancreas (D, negative control) lysates (in RIPA buffer) stained with ARG63188 anti-RACGAP1 / MgcRacGAP antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63188 anti-RACGAP1 / MgcRacGAP antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed MCF7 cells permeabilized with 0.5% Triton. Cells were stained with ARG63188 anti-RACGAP1 / MgcRacGAP antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
