ARG42510
anti-RAB5A antibody
anti-RAB5A antibody for ICC/IF,Western blot and Dog,Human,Monkey,Mouse,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes RAB5A |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RAB5A |
| 抗原物种 | Mouse |
| 抗原 | Purified recombinant peptide within aa. 115 to the C-terminus of Mouse RAB5A. |
| 偶联标记 | Un-conjugated |
| 別名 | Ras-related protein Rab-5A; RAB5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | SW948, NIH/3T3, MCF7, MDCK and HeLa | ||||||
| 实际分子量 | ~ 27 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 4 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RAB5A |
| 全名 | RAB5A, member RAS oncogene family |
| 生物功能 | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Endosome membrane. Note=Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). [UniProt] |
| 预测分子量 | 24 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG42510 anti-RAB5A antibody ICC/IF image
Immunofluorescence: Hepa1-6 cells were fixed with 4% PFA. Cells were stained with ARG42510 anti-RAB5A antibody (green) at 1:50 dilution. Nuclear staining (blue).
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ARG42510 anti-RAB5A antibody WB image
Western blot: 50 µg of SW948, NIH/3T3, MCF7, MDCK and HeLa cell lysates stained with ARG42510 anti-RAB5A antibody at 1:2500 dilution.
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ARG42510 anti-RAB5A antibody IHC-P image
Immunohistochemistry: Mouse Intestine stained with ARG42510 anti-RAB5A antibody at 1:250 dilution.
