ARG54014
anti-Pyruvate Dehydrogenase E1 alpha subunit antibody
anti-Pyruvate Dehydrogenase E1 alpha subunit antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Pyruvate Dehydrogenase E1 alpha subunit |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | Pyruvate Dehydrogenase E1 alpha subunit |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A; EC 1.2.4.1; PDHE1-A type I; PDHCE1A; PDHAD; PDHA |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08559 Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochond Swiss-port # P35486 Mouse Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochond |
|---|---|
| 基因名称 | PDHA1 |
| 全名 | pyruvate dehydrogenase (lipoamide) alpha 1 |
| 背景介绍 | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). |
| 生物功能 | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt] |
| 细胞定位 | Mitochondrion matrix |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity). |
检测图片 (2) Click the Picture to Zoom In
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ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody (green) at 1:100 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody WB image
Western blot: 30 µg of 293T and HeLa cell lysates stained with ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody at 1:1000 dilution.
客户反馈
Good
anti-Pyruvate Dehydrogenase E1 alpha subunit antibody
Application：WB
Sample：HepG2
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Pyruvate Dehydrogenase E1 alpha subunit antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
