ARG64474
anti-Pyruvate Carboxylase antibody
anti-Pyruvate Carboxylase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Pyruvate Carboxylase |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | FACS, IHC-P |
| 特异性 | All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Pyruvate Carboxylase |
| 抗原物种 | Human |
| 抗原 | C-KFKEVKKAYVEANQ |
| 偶联标记 | Un-conjugated |
| 別名 | PCB; Pyruvic carboxylase; EC 6.4.1.1; Pyruvate carboxylase, mitochondrial |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P11498 Human Pyruvate carboxylase, mitochondrial |
|---|---|
| 背景介绍 | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 130 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64474 anti-Pyruvate Carboxylase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64474 anti-Pyruvate Carboxylase antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64474 anti-Pyruvate Carboxylase antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64474 anti-Pyruvate Carboxylase antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
