ARG41418

anti-Protein C antibody

anti-Protein C antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes Protein C
反应物种 Hu
应用 FACS, IHC-P
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Protein C
抗原物种 Human
抗原 Synthetic peptide corresponding to aa. 446-461 of Human Protein C. (HGHIRDKEAPQKSWAP)
偶联标记 Un-conjugated
別名 EC 3.4.21.69; Blood coagulation factor XIV; PC; THPH3; Vitamin K-dependent protein C; THPH4; APC; Autoprothrombin IIA; PROC1; Anticoagulant protein C

应用说明

应用建议
应用 推荐稀释比
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Thimerosal and 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5624 Human PROC

Swiss-port # P04070 Human Vitamin K-dependent protein C

基因名称 PROC
全名 protein C (inactivator of coagulation factors Va and VIIIa)
背景介绍 This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]
生物功能 Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. [UniProt]
细胞定位 Secreted. Golgi apparatus. Endoplasmic reticulum. [UniProt]
预测分子量 52 kDa
翻译后修饰 The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.

N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG41418 anti-Protein C antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41418 anti-Protein C antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG41418 anti-Protein C antibody FACS image

    Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG41418 anti-Protein C antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.