ARG55780

Name: anti-Proteasome 20S LMP2 antibody
Brand: arigo Biolaboratories
SKU: ARG55780
Price: 399 USD
Availability: InStock
Rating: 3.0 (1 reviews)

anti-Proteasome 20S LMP2 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

50 μl

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes Proteasome 20S LMP2
反应物种	Hu
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	Proteasome 20S LMP2
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 193-219 (C-terminus) of Human Proteasome 20S LMP2.
偶联标记	Un-conjugated
別名	beta1i; Proteasome subunit beta type-9; Really interesting new gene 12 protein; Proteasome chain 7; Multicatalytic endopeptidase complex chain 7; Proteasome subunit beta-1i; Macropain chain 7; RING12; EC 3.4.25.1; LMP2; PSMB6i; Low molecular mass protein 2

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
IHC-P	1:50 - 1:100
WB	1:500 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A and immunogen peptide.
缓冲液	PBS and 0.09% (W/V) Sodium azide.
抗菌剂	0.09% (W/V) Sodium azide.
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 5698 Human PSMB9 Swiss-port # P28065 Human Proteasome subunit beta type-9
基因名称	PSMB9
全名	proteasome (prosome, macropain) subunit, beta type, 9
背景介绍	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]
生物功能	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. [UniProt]
细胞定位	Cytoplasm. Nucleus
预测分子量	23 kDa
翻译后修饰	Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

检测图片 (3) Click the Picture to Zoom In

ARG55780 anti-Proteasome 20S LMP2 antibody WB image

Western blot: 30 µg of A431 cell lysate stained with ARG55780 anti-Proteasome 20S LMP2 antibody at 1:1000 dilution.
ARG55780 anti-Proteasome 20S LMP2 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate carcinoma stained with ARG55780 anti-Proteasome 20S LMP2 antibody.
ARG55780 anti-Proteasome 20S LMP2 antibody FACS image

Flow Cytometry: HL60 cells stained with ARG55780 anti-Proteasome 20S LMP2 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.