ARG23771
anti-Plexin A1 antibody
anti-Plexin A1 antibody for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Plexin A1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | This antibody detects a ~220 kDa protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Plexin A1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (coupled to carrier protein) around the semaphorin domain (N-terminal half) of Human Plexin A1. This sequence is highly conserved in Rat and Mouse Plexin A1, and has low homology to other Plexin family members. |
| 偶联标记 | Un-conjugated |
| 別名 | Semaphorin receptor NOV; PLEXIN-A1; PLXN1; NOV; Plexin-A1; NOVP |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 220 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PLXNA1 |
| 全名 | plexin A1 |
| 生物功能 | Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm (By similarity). [UniProt] |
| 预测分子量 | 211 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG23771 anti-Plexin A1 antibody ICC/IF image
Immunofluorescence: Double labeling using ARG23771 anti-Plexin A1 antibody in COS7 cells mock transfected (A,D) or transfected with Myc-tagged Mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of primary antibodies (C,F).
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ARG23771 anti-Plexin A1 antibody WB image
Western blot: COS7 cells transfected with mouse Myc-tagged Plexin A1 and neonatal Rat brain (from left to right) stained with ARG23771 anti-Plexin A1 antibody.
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ARG23771 anti-Plexin A1 antibody IP image
Immunoprecipitation: Myc-Plexin A1 transfected COS7 immunoprecipitated by ARG23771 anti-Plexin A1 antibody and stained with anti-Myc antibody.
