ARG63589
anti-Pleckstrin antibody
anti-Pleckstrin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Pleckstrin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Pleckstrin |
| 抗原物种 | Human |
| 抗原 | C-EWIKAIQMASRTGK |
| 偶联标记 | Un-conjugated |
| 別名 | P47; p47; Pleckstrin; Platelet 47 kDa protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PLEK |
| 全名 | pleckstrin |
| 生物功能 | Major protein kinase C substrate of platelets. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 40 kDa |
检测图片 (6) Click the Picture to Zoom In
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ARG63589 anti-Pleckstrin antibody WB image
Western Blot: Human PBMC lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63589 anti-Pleckstrin antibody at 0.05 µg/ml dilution.
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ARG63589 anti-Pleckstrin antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63589 anti-Pleckstrin antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63589 anti-Pleckstrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human vessel (platelets) tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63589 anti-Pleckstrin antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63589 anti-Pleckstrin antibody WB image
Western blot: 35 µg of Human parathyroid (A) and HepG2 (B, negative control) lysates (in RIPA buffer) stained with ARG63589 anti-Pleckstrin antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
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ARG63589 anti-Pleckstrin antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63589 anti-Pleckstrin antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63589 anti-Pleckstrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63589 anti-Pleckstrin antibody at 3.75 µg/ml dilution followed by AP-staining.
