ARG63633

anti-Pirin antibody

anti-Pirin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Gene Regulation antibody

概述

产品描述 Goat Polyclonal antibody recognizes Pirin
反应物种 Hu
预测物种 Ms
应用 IHC-P, WB
特异性 Variants (NP_003653.1; NP_001018119.1) encode the same protein
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Pirin
抗原物种 Human
抗原 GSSKKVTLSVLSREC
偶联标记 Un-conjugated
別名 EC 1.13.11.24; Probable quercetinase; Pirin; Probable quercetin 2,3-dioxygenase PIR

应用说明

应用建议
应用 推荐稀释比
IHC-P5 µg/ml
WB0.3 - 1.0 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 8544 Human PIR

Swiss-port # O00625 Human Pirin

背景介绍 This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
研究领域 Controls and Markers antibody; Gene Regulation antibody
预测分子量 32 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG63633 anti-Pirin antibody WB image

    Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG63633 anti-Pirin antibody at 0.3 µg/ml dilution.

  • ARG63633 anti-Pirin antibody WB image

    Western Blot: 1). Mock transfection; 2) PIR (RC223111) expressing plasmid transfected HEK293 cell lysate standed with ARG63633 anti-Pirin antibody

  • ARG63633 anti-Pirin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63633 anti-Pirin antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63633 anti-Pirin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63633 anti-Pirin antibody at 5 µg/ml dilution followed by AP-staining.