ARG44472

anti-Pirin antibody

anti-Pirin antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes Pirin
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Pirin
抗原物种 Human
抗原 Human Pirin recombinant protein
偶联标记 Un-conjugated
別名 PIR; Pirin; Probable Quercetin 2,3-Dioxygenase PIR; Pirin (Iron-Binding Nuclear Protein); Probable Quercetinase

应用说明

应用建议
应用 推荐稀释比
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
WB0.1-0.25 µg/ml
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

生物信息

数据库连接

GeneID: 363465 Rat PIR

GeneID: 69656 Mouse PIR

GeneID: 8544 Human PIR

基因名称 PIR
全名 Pirin
背景介绍 This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.
生物功能 Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity.
细胞定位 Cytoplasm, Nucleus
预测分子量 32 kDa

检测图片 (6) Click the Picture to Zoom In

  • ARG44472 anti-Pirin antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG44472 anti-Pirin antibody at 5 μg/mL dilution.

  • ARG44472 anti-Pirin antibody WB image

    Western blot: HepG2 and HeLa stained with ARG44472 anti-Pirin antibody at 0.5 μg/mL dilution.

  • ARG44472 anti-Pirin antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44472 anti-Pirin antibody at 1 μg/10^6 cells dilution.

  • ARG44472 anti-Pirin antibody WB image

    Western blot: RH-35 stained with ARG44472 anti-Pirin antibody at 0.5 μg/mL dilution.

  • ARG44472 anti-Pirin antibody WB image

    Western blot: NIH/3T3 stained with ARG44472 anti-Pirin antibody at 0.5 μg/mL dilution.

  • ARG44472 anti-Pirin antibody WB image

    Western blot: COS-7 stained with ARG44472 anti-Pirin antibodyat 0.5 μg/mL dilution.