ARG63086

anti-Phosphotyrosine antibody [P-TYR-01]

anti-Phosphotyrosine antibody [P-TYR-01] for Flow cytometry,ICC/IF,Western blot and All

Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

概述

产品描述 Mouse Monoclonal antibody [P-TYR-01] recognizes Phosphotyrosine
反应物种 All
应用 FACS, ICC/IF, WB
特异性 The clone P-Tyr-01 detects tyrosine phosphorylation in activated cells.
宿主 Mouse
克隆 Monoclonal
克隆号 P-TYR-01
同位型 IgG1
靶点名称 Phosphotyrosine
抗原 Phosphotyrosine conjugated to bovine serum albumin.
偶联标记 Un-conjugated

应用说明

应用建议
应用 推荐稀释比
FACS2 - 5 µg/ml
ICC/IFAssay-dependent
WB1 - 2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from ascites by protein-A affinity chromatography.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

研究领域 Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

检测图片 (1) Click the Picture to Zoom In

  • ARG63086 anti-Phosphotyrosine antibody [P-TYR-01] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG63086 anti-Phosphotyrosine antibody [P-TYR-01] (green).
    Actin was stained with phalloidin-Alexa Fluor-567 (red), DNA was stained with DAPI (blue).

克隆号文献

Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization.

Saxinger C et al.
BMC Immunol.,  (2005)

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Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.

Brdicka T et al.
J Exp Med.,  (2000)

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T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains.

Brdiĉka T et al.
Biochem Biophys Res Commun.,  (1998)

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Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.

Cebecauer M et al.
Biochem Biophys Res Commun.,  (1998)

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