ARG57186
anti-Phosphothreonine antibody [RM102]
anti-Phosphothreonine antibody [RM102] for ICC/IF,Immunoprecipitation,Western blot and Other
概述
| 产品描述 | Rabbit Monoclonal antibody [RM102] recognizes Phosphothreonine |
|---|---|
| 反应物种 | Other |
| 应用 | ICC/IF, IP, WB |
| 特异性 | This antibody reacts to threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides. |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | RM102 |
| 同位型 | IgG |
| 靶点名称 | Phosphothreonine |
| 抗原物种 | Others |
| 抗原 | Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol and 1% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (4) Click the Picture to Zoom In
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ARG57186 anti-Phosphothreonine antibody [RM102] ICC/IF image
Immunofluorescence: Serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, stained with ARG57186 anti-Phosphothreonine antibody [RM102] at 1:500 dilution (followed by PE-conjugated secondary antibody, red) and DAPI (blue).
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ARG57186 anti-Phosphothreonine antibody [RM102] WB image
Western blot: serum-starved A431 cells non-treated or treated with Calyculin A / Okadaic Acid, stained with ARG57186 anti-Phosphothreonine antibody [RM102] at 1:2000 dilution.
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ARG57186 anti-Phosphothreonine antibody [RM102] IP image
Immunoprecipitation: Calyculin A / Okadaic Acid treated A431 cells by ARG57186 anti-Phosphothreonine antibody [RM102] at 1:500 dilution, and then blotted with the same antibody. 1) Whole cell lysate control, 2) IP by Rabbit IgG control, and 3) IP by ARG57186 anti-Phosphothreonine antibody [RM102].
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ARG57186 anti-Phosphothreonine antibody [RM102] Specificity test image
ARG57186 anti-Phosphothreonine antibody [RM102] recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.
