ARG23898
anti-Phosphoserine / threonine antibody
anti-Phosphoserine / threonine antibody for ELISA,ICC/IF,Immunoprecipitation,Western blot and Other
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Phosphoserine / threonine |
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| 反应物种 | Other |
| 应用 | ELISA, ICC/IF, IP, WB |
| 特异性 | This antibody detects many serine or threonine phosphorylated proteins by WB, ICC and ELISA. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Serine / Threonine |
| 抗原物种 | Human |
| 抗原 | A panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 纯化 | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
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| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
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检测图片 (3) Click the Picture to Zoom In
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ARG23898 anti-Phosphoserine / threonine antibody WB image
Western blot: A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was stained with ARG23898 anti-Phosphoserine / threonine antibody at 1:1000 dilution.
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ARG23898 anti-Phosphoserine / threonine antibody ICC/IF image
Immunofluorescence: Control (left) and calyculin A-treated (right) A431 cells were stained with ARG23898 anti-Phosphoserine / threonine antibody.
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ARG23898 anti-Phosphoserine / threonine antibody binding to phosphopeptides
Bar graph showing ARG23898 anti-Phosphoserine / threonine antibody binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
