ARG20543

anti-Phosphoserine antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μl

389

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes Phosphoserine
反应物种	Other
应用	ELISA, ICC/IF, IHC-P, IP, WB
特异性	Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
宿主	Rabbit
克隆	Polyclonal
靶点名称	Phosphoserine
抗原	KLH-conjugated Phosphoserine, and phosvitin mixture
偶联标记	Un-conjugated

应用说明

应用建议

应用	推荐稀释比
ELISA	1:250
ICC/IF	1:50
IHC-P	1:250
IP	1:100
WB	1:500

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Rabbit immunoglobulin
缓冲液	PBS, 50% Glycerol and 0.09% Sodium azide
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol
浓度	250 µg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
研究领域	Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

背景介绍

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.

研究领域

Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

检测图片 (2) Click the Picture to Zoom In

ARG20543 anti-Phosphoserine antibody ICC/IF image

Immunofluorescence: HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20543 anti-Phosphoserine antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20543 anti-Phosphoserine antibody. Right: Composite.
ARG20543 anti-Phosphoserine antibody WB image

Western blot: the phosphorylated proteins with UV-treated cell lysates (mouse spleen cell) stained with ARG20543 anti-Phosphoserine antibody. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1 μM okadaic acid).