ARG45953

anti-Phosphoribosyl pyrophosphate amidotransferase antibody

anti-Phosphoribosyl pyrophosphate amidotransferase antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes Phosphoribosyl pyrophosphate amidotransferase
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Phosphoribosyl pyrophosphate amidotransferase
抗原物种 Human
抗原 Recombinant protein containing to human Phosphoribosyl pyrophosphate amidotransferase.
偶联标记 Un-conjugated
別名 Phosphoribosyl Pyrophosphate Amidotransferase; PPAT; GPAT; Amidophosphoribosyltransferase; ATase; EC 2.4.2.14; Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 57 kDa

属性

形式 Liquid
纯化 Affinity chromatography purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 117544 Rat PPAT

GeneID: 5471 Human PPAT

Swiss-port # P35433 Rat Amidophosphoribosyltransferase

Swiss-port # Q06203 Human Amidophosphoribosyltransferase

基因名称 PPAT
全名 Phosphoribosyl Pyrophosphate Amidotransferase
背景介绍 The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Jul 2008]
生物功能 Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine. [UniProt]
细胞定位 Cytosol. [UniProt]
预测分子量 57 kDa
翻译后修饰 Acetylation. [UniProt]. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody at 5 μg/ml dilution.

  • ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody WB image

    Western blot: 293T stained with ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody at 0.5 μg/ml dilution.

  • ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody FACS image

    Flow Cytometry: K562 stained with ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody at 1 µg/10^6 cells dilution.

  • ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody WB image

    Western blot: PC-12 stained with ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody at 0.5 μg/ml dilution.

  • ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody WB image

    Western blot: RAW264.7 stained with ARG45953 anti-Phosphoribosyl pyrophosphate amidotransferase antibody at 0.5 μg/ml dilution.