ARG57055
anti-Peroxiredoxin 5 antibody [6A10]
anti-Peroxiredoxin 5 antibody [6A10] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [6A10] recognizes Peroxiredoxin 5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6A10 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Peroxiredoxin 5 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 53-214 of Human Peroxiredoxin 5. |
| 偶联标记 | Un-conjugated |
| 別名 | PMP20; EC 1.11.1.15; Thioredoxin peroxidase PMP20; ACR1; Prx-V; PLP; PRXV; PRDX6; HEL-S-55; B166; Thioredoxin reductase; Peroxiredoxin-5, mitochondrial; AOEB166; Peroxiredoxin V; SBBI10; prx-V; Antioxidant enzyme B166; Alu corepressor 1; TPx type VI; Peroxisomal antioxidant enzyme; Liver tissue 2D-page spot 71B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRDX5 |
| 全名 | peroxiredoxin 5 |
| 背景介绍 | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. [UniProt] |
| 预测分子量 | 22 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57055 anti-Peroxiredoxin 5 antibody [6A10] FACS image
Flow Cytometry: HeLa cell line stained with ARG57055 anti-Peroxiredoxin 5 antibody [6A10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57055 anti-Peroxiredoxin 5 antibody [6A10] WB image
Western blot: 1) 50 ng of Recombinant PRDX5 protein, 40 µg of 2) MCF-7 cell lysate, 3) A549 cell lysate, 4) HeLa cell lysate, 5) 293T cell lysate, 6) Jurkat cell lysate stained with ARG57055 anti-Peroxiredoxin 5 antibody [6A10] at 1:1000.
