ARG57066
anti-Peroxiredoxin 3 antibody [1F8]
anti-Peroxiredoxin 3 antibody [1F8] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1F8] recognizes Peroxiredoxin 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1F8 |
| 同位型 | IgG3, kappa |
| 靶点名称 | Peroxiredoxin 3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 63-256 of Human Peroxiredoxin3. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.11.1.15; Antioxidant protein 1; Prx-III; SP-22; AOP1; AOP-1; MER5; HBC189; PRO1748; Peroxiredoxin-3; Thioredoxin-dependent peroxide reductase, mitochondrial; prx-III; Peroxiredoxin III; Protein MER5 homolog |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30048 Human Thioredoxin-dependent peroxide reductase, mitochondrial |
|---|---|
| 基因名称 | PRDX3 |
| 全名 | peroxiredoxin 3 |
| 背景介绍 | This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014] |
| 生物功能 | Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. [UniProt] |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Phosphorylated by LRRK2; phosphorylation reduces perodixase activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG57066 anti-Peroxiredoxin 3 antibody [1F8] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57066 anti-Peroxiredoxin 3 antibody [1F8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57066 anti-Peroxiredoxin 3 antibody [1F8] WB image
Western blot: 1) 50 ng of Recombinant protein, 40 µg of 2) HeLa cell lysate, 3) HepG2 cell lysate, 4) TF1 cell lysate, 5) U87MG cell lysate, 6) Raji cell lysate, 7) 293T cell lysate, 8) Jurkat cell lysate, 9) MCF7 cell lysate stained with ARG57066 anti-Peroxiredoxin 3 antibody [1F8] at 1:1000.
