ARG23249
anti-Pan B-Cell antibody [CVS36] (PE)
anti-Pan B-Cell antibody [CVS36] (PE) for Flow cytometry and Horse
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [CVS36] recognizes Pan B-Cells Mouse anti Horse Pan B-Cells, clone CVS36 is a monoclonal antibody directed against equine Ig light chains. Characterization studies demonstrated that clone CVS36 bound 100% of CD5-ve peripheral blood lymphocytes and recognized all equine B-cells (Lunn et al. 1998) As the antigen recognized by clone CVS36 appears to be present on the surface of all equine B-cells it is therefore is a reagent that can be used as a pan B-cell marker for domestic horses (Breathnach et al. 2005). Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the Mouse anti Human CD79a antibody, (clone HM57), an overview of which may be found in the report of the second equine leucocyte antigen workshop (Lunn et al. 1998). With specificity for equine Ig light chains, clone CVS36 may be used to detect all equine immunoglobulin classes and subclasses in ELISA applications (Lunn et al. 1998). |
|---|---|
| 反应物种 | Hrs |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CVS36 |
| 同位型 | IgG1 |
| 靶点名称 | Pan B-Cell |
| 抗原物种 | Horse |
| 抗原 | Purified Equine Ig. |
| 偶联标记 | PE |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1) Click the Picture to Zoom In
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ARG23249 anti-Pan B-Cell antibody [CVS36] (PE) FACS image
Flow Cytometry: Horse peripheral blood lymphocytes stained with ARG23249 anti-Pan B-Cell antibody [CVS36] (PE).
