ARG54439
anti-PUMA antibody
anti-PUMA antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PUMA |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody recognizes human and mouse PUMA (23kDa). A lower molecular weight band at 16kDa is detected in MOLT4 and U937 cell lysates and probably represents the PUMA- protein. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PUMA |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa 180-193 of human PUMA-alpha (accession no NP_055232). This sequence is identical in human PUMA-alpha and PUMA-beta . |
| 偶联标记 | Un-conjugated |
| 別名 | PUMA; p53 up-regulated modulator of apoptosis; JFY-1; JFY1; Bcl-2-binding component 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 and NIH/3T3 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BBC3 |
| 全名 | BCL2 binding component 3 |
| 背景介绍 | PUMA (p53 Upregulated Modulator of Apoptosis) is a pro-apoptotic Bcl-2 family member that encodes two BH3 domain-containing proteins, PUMA- and PUMA- . PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53. |
| 生物功能 | Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 26 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54439 anti-PUMA antibody WB image
Western Blot: H:K562; M:NIH/3T3 stained with ARG54439 anti-PUMA antibody at 2 μg/ml dilution.
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ARG54439 anti-PUMA antibody ICC/IF image
Immunofluorescence: K562 stained with ARG54439 anti-PUMA antibody at 1 μg/ml dilution.
