概述

产品描述	Rabbit Polyclonal antibody recognizes PU.1
反应物种	Hu
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	PU.1
抗原物种	Human
抗原	Synthetic peptide derived from Human PU.1.
偶联标记	Un-conjugated
別名	Transcription factor PU.1; SPI-1; OF; SPI-A; PU.1; SFPI1; 31 kDa-transforming protein

应用说明

应用建议	应用 推荐稀释比 FACS 1:60 IHC-P 1:50 - 1:200 WB 1:1000 - 1:5000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	Daudi
实际分子量	~ 42 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 6688 Human SPI1 Swiss-port # P17947 Human Transcription factor PU.1
基因名称	SPI1
全名	Spi-1 proto-oncogene
背景介绍	This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能	Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). [UniProt]
预测分子量	31 kDa

检测图片 (1) Click the Picture to Zoom In