ARG41973
anti-PTBP2 antibody
anti-PTBP2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PTBP2 |
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| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PTBP2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to M1-A504 of Human PTBP2. |
| 偶联标记 | Un-conjugated |
| 別名 | Polypyrimidine tract-binding protein 2; nPTB; PTBLP; Neurally-enriched homolog of PTB; Neural polypyrimidine tract-binding protein; brPTB; PTB-like protein |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 57 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PTBP2 |
| 全名 | polypyrimidine tract binding protein 2 |
| 背景介绍 | The protein encoded by this gene binds to intronic polypyrimidine clusters in pre-mRNA molecules and is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein (PTB) but most of its isoforms are expressed primarily in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 57 kDa |
检测图片 (9) Click the Picture to Zoom In
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ARG41973 anti-PTBP2 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG41973 anti-PTBP2 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse cardiac muscle tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41973 anti-PTBP2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. MDA-MB-453, U2OS, THP-1, K562, U-937, HEK293 and HeLa whole cell lysates stained with ARG41973 anti-PTBP2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody FACS image
Flow Cytometry: HeLa cells were blocked with 10% normal goat serum and then stained with ARG41973 anti-PTBP2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41973 anti-PTBP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41973 anti-PTBP2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41973 anti-PTBP2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41973 anti-PTBP2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat testis, Mouse testis, Mouse thymus and Mouse HEPA1-6 whole cell lysates stained with ARG41973 anti-PTBP2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41973 anti-PTBP2 antibody FACS image
Flow Cytometry: THP-1 cells were blocked with 10% normal goat serum and then stained with ARG41973 anti-PTBP2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
