ARG55445
anti-PSME2 antibody
anti-PSME2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PSME2 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PSME2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 210-239 (C-terminus) of Human PSME2. |
| 偶联标记 | Un-conjugated |
| 別名 | 11S regulator complex subunit beta; REG-beta; PA28beta; Proteasome activator 28 subunit beta; Activator of multicatalytic protease subunit 2; PA28b; PA28B; Proteasome activator complex subunit 2; REGbeta |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P97372 Mouse Proteasome activator complex subunit 2 Swiss-port # Q9UL46 Human Proteasome activator complex subunit 2 |
|---|---|
| 基因名称 | PSME2 |
| 全名 | proteasome (prosome, macropain) activator subunit 2 (PA28 beta) |
| 背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13. [provided by RefSeq, Jul 2008] |
| 生物功能 | Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 27 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG55445 anti-PSME2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse hepatocarcinoma stained with ARG55445 anti-PSME2 antibody.
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ARG55445 anti-PSME2 antibody WB image
Western blot: 35 µg of HepG2 cell lysate stained with ARG55445 anti-PSME2 antibody at 1:1000 dilution.
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ARG55445 anti-PSME2 antibody FACS image
Flow Cytometry: HL-60 cells stained with ARG55445 anti-PSME2 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
